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Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach
Oligonucleotide-directed in vitro mutagenesis is an established tool for the inv
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Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Folli
Human induced pluripotent stem cells (hiPSCs) provide a unique experimental reag
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Structural Studies of MAP Kinase Cascade Components
MAPK cascade components have been the subject of structural analysis, advancing
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RACE实验是什么？【分子生物学】
RACE实验的类型多样，包括经典RACE、Cap-switching RACE、环形RACE等。每种类型都有其独特的原理和应用场景，如经典RACE主要用于克隆c
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Preparation of DRV Liposomes
Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild
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Procedures to Evaluate the Importance of Dietary Polyamines
Polyamines not only play vital physiological functions including modulating tran
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RACE实验提高扩增特异性和效率的新方法
一、优化引物设计选择合适的引物长度和GC含量：引物长度建议为23~28个核苷酸，GC含量为50%~70%。较高的GC比例和Tm值（65℃，若>70℃使
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RACE实验与基因表达分析
RACE实验与基因表达分析~首先还是从RACE实验的原理开始介绍，即cDNA末端快速扩增技术（Rapid Amplification of cDNA Ends）
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cDNA末端快速扩增技术（RACE）在基因克隆中的实践
一、RACE技术概述RACE技术，即Rapid Amplification of cDNA Ends，是一种基于PCR技术，从已知的部分cDNA序列出发，快速扩
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脊髓星形胶质细胞机械性损伤的体外培养模型
1.材料与方法 取新生的wistar 大鼠脊髓,剪碎,0125 %胰蛋白酶消化1h ,用含血清的DMEM培养液终止胰蛋白酶的作用; 而后吹打成散在的单个细胞,进
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探索RACE实验在疾病相关基因研究中的应用价值
1}克隆疾病相关基因的全长序列RACE实验能够高效地从mRNA的3'端或5'端扩增出未知的cDNA序列，从而填补基因序列的空白。在疾病相关基因研
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RACE实验优化策略：提升实验成功率的实用技巧
一、引物设计与选择⑴引物长度与GC含量：引物长度建议控制在23-28个核苷酸之间，不超过30个。GC含量应保持在50%-70%之间，以确保引物的稳定性和扩增
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mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridizatio
Two methods are commonly employed to characterize the spatiotemporal aspect of g
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RACE实验有哪些发展趋势？
一、技术本身的优化与创新【提高特异性和灵敏度】：随着分子生物学技术的不断发展，RACE实验将不断优化其引物设计、反转录和PCR扩增策略，以提高实验的特异性和
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Directing Human Embryonic Stem Cells to a Retinal Fate
Substantial progress has been made in the development of methods to direct embry
