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Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests
Infants born prematurely have an enhanced intestinal permeability compared to he
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Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interac
The dynamics of proteins play a key role in the organization and control of nucl
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Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
Cloning using somatic cell nuclear transfer (SCNT) may be a useful tool for cons
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Cryo-electron Tomography of Microtubules Assembled In Vitro from Purified Components
Cryo-electron tomography of vitrified specimens allows visualization of thin bio
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TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannhei
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RACE实验在基因克隆中的应用:从理论到实践的跨越
一、RACE实验的理论基础RACE技术是一种基于mRNA反转录和PCR技术的分子生物学方法。其原理是利用已知的部分cDNA序列,通过设计特异性引物,结合逆转录和
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Quantification of Protein Carbonylation
Protein carbonylation is the most commonly used measure of oxidative modificatio
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Plasmid DNA Purification Via the Use of a Dual Affinity Protein
Methods are presented for the production, affinity purification and analysis of
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RACE实验深度探索:从原理到应用的全面解析
——RACE技术的原理 RACE技术的核心在于利用已知的部分cDNA序列,通过逆转录和PCR扩增,获得完整的cDNA的5′和3′端序列。这一过程分为3′RAC
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Affinity-Based Assays for the Identification and Quantitative Evaluation of Noncovalent Poly(ADP-Rib
Poly(ADP-ribose) polymerases have been linked to several cellular functions, mos
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Regulation of MAP Kinase Signaling by Calcium
Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellul
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Chemokine Receptor Signaling and HIV Infection
The primary function of HIV-1 binding to its chemokine coreceptors is to mediate
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模板组装(template assembly)
由模板指导,在一系列酶的催化下,合成新的、与模板完全相同的分子。这是细胞内一种极其重要的组装方式, DNA和RNA的分子组装就属于此类。
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Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
Protein phosphorylation is a versatile posttranslational modification that can r
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考马斯亮蓝染色原理
一、染料特性考马斯亮蓝G-250是常用的考马斯亮蓝染料之一。在游离状态下,它通常呈现红色,并且具有两种形态:一种是水溶性的红色形态,另一种是疏水性的蓝色形态。这