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Sequence-Based Typing of HLA: An Improved Group-Specific Full-Length Gene Sequencing Approach
Matching for HLA at the allele level is crucial for stem cell transplantation. T
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Overview of Cell Synchronization
Widespread interest in cell synchronization is maintained by the studies of cont
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考马斯亮蓝染色步骤全解析
1）前期准备电泳结束：首先，通过SDS-PAGE电泳将蛋白质进行分离，电泳结束后，取出凝胶。清洗凝胶：将凝胶放入加有超纯水的平皿中润洗，以去除残留的电泳
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Isolating mRNA and Small Noncoding RNAs from Human Sperm
The isolation of spermatozoal RNA is a challenging procedure due to the intrinsi
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Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
Cytoplasmic egg extracts from the frog Xenopus laevis represent a powerful cell-
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考马斯亮蓝测蛋白浓度步骤
1.准备试剂与器材考马斯亮蓝G-250试剂：通常是将100mg考马斯亮蓝G-250溶于50mL的95%乙醇中，再加入适量的85%磷酸，最后用蒸馏水稀释至1L
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Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells
Heterogeneity of stem cell populations is a well-known but poorly characterized
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养大鼠平滑肌细胞的一点心得
我养了快半年的大鼠气道平滑肌细胞，有一点体会，特发上来和各位战友交流一下，互相进步。 壹． 器械 显微器械：眼科剪一把，直弯眼科镊各一把，细结镊一把，手术刀一
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考马斯亮蓝染色原理与传统染色
●配方组成①考马斯亮蓝R250：0.5克。考马斯亮蓝R250是主要的染料成分，能够与蛋白质结合并显色。②异丙醇：125毫升。异丙醇作为有机溶剂，有助于染
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Viral Infection Protocols
This chapter describes the protocol for preparation of recombinant adenoviruses
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与传统染色相比，考马斯亮蓝染色有哪些具体优势？
【一】灵敏度高考马斯亮蓝染色法的灵敏度显著高于传统染色方法，如Lowry法等。其最低蛋白质检测量可达微克级，甚至能检出0.1μg的蛋白量，这对于微量蛋白质的检测
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Purification of Drosophila Protein Complexes for Mass Spectrometry
Drosophila melanogaster is one of the best characterized model systems for genet
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Rhythmic Conidiation in Neurospora crassa
In the filamentous fungus Neurospora crassa the production of asexual spores (co
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考马斯亮蓝染色后，如何保存凝胶？
1）置于保存液中保存染色并脱色至获得清晰的蓝色条带和干净背景后，可将凝胶放置在7%的乙酸中保存。这种方法可以有效地固定凝胶上的蛋白质，防止其降解或脱落，同时保持
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Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic
The epidermis is a complex tissue composed principally of differentiated keratin
