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慢病毒感染后72小时，效率低，细胞已经长满，有什么办法补救？
在慢病毒感染后72小时感染效率较低、细胞已经长满的情况下，可以尝试以下几个措施来提高感染效率并优化实验条件：细胞传代或重新播种：如果细胞已经长满（汇合度较高），
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Behavioral Methods for the Study of the RasERK Pathway in Memory Formation and Consolidation: Passiv
Memory is a high-level brain function that enables organisms to adapt their beha
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Bone Marrow-Derived Mesenchymal Progenitor Cells
Primary monolayer cultures of marrow stromal cells, as they are referred to in t
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Skeletal Morphogenesis
The formation of a skeleton with its numerous bones of various shapes and sizes
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Function of the Wingless Signaling Pathway in Drosophila
Signaling by the wingless pathway has been shown to govern numerous developmenta
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Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion
Mapping DNase I hypersensitive sites can often localize the control regions in a
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Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcri
The assessment of the viral titer is an important step in optimizing and reprodu
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Developmental Genetics in Xenopus tropicalis
The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new
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Using 3D Culture to Investigate the Role of Mechanical Signaling in Keratinocyte Stem Cells
The ability to grow keratinocyte stem cells (KSCs) in 3D culture is an important
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Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
Replication intermediates can be separated on agarose gels in two dimensions to
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Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine
Regulation of protein activity through the oxidation and reduction of cysteines
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Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells
The ability to stably introduce genetic material into primate embryonic stem (ES
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Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin-16
The integral tight junction protein Claudin-16 (Cldn16) is predominantly express
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Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage
Many research groups are engaged in using human pluripotent stem cells (hPSCs) t
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Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies
Two techniques have been described that use the polymerase chain reaction (PCR)
