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End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA
Polymerase chain reaction (PCR) is an important qualitative procedure in the rou
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Pharmacology, Biodistribution, and Efficacy of GPCR-Based Pepducins in Disease Models
G protein-coupled receptors (GPCR) are a superfamily of receptors that are vital
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2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics
2D- and 3D-Dynamic Image Analysis Systems (2D- and 3D-DIAS) for quantitative ana
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In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Adult bone marrow stem cell is an ideal target for gene therapy of genetic disea
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Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clo
Owing to the nearly limitless diversity of immunoglobulin (Ig) variable-region g
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Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry
Imaging mass spectrometry (IMS) provides a unique method to probe for chemical d
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Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
In this chapter, we describe our methods to evaluate the dynein heavy chain gene
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Quantification of Genetically Controlled Cell Death in Budding Yeast
Yeast are the foremost genetic model system. With relative ease, entire chemical
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The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
The osteoclast is a large multinucleate cell formed from the fusion of mono-nucl
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细胞生物基本方法：特殊培养法
特殊培养法 1 ）二倍体细胞培养法 二倍体细胞培养法与一般培养相同，关键在于传代，其传代程序为：1. 吸除旧培养液注入另瓶中。2. 用温 B
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TUNEL PROCEDURE IN BOVINE EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered pa
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Electrofusion of Mammalian Cells
Electrofusion has developed into an extremely efficient method for the fusion of
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Nuclear Reprogramming: An Overview
Nuclear reprogramming is an intriguing phenomenon, in which specialized somatic
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双线期(diplotene stage)
前期I的第四个阶段，此期染色体长度进一步变短，联会复合体因发生去组装而逐渐消失，紧密配对的同源染色体相互分开，而在非姊妹染色单体之间的某些部位上，可见其相互间有
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Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic
A variety of assays, and rationales for their use, exist to monitor viability an
