-
核纤层蛋白(nuclear lamina protein)
脊椎动物细胞中有三种类型的核纤层蛋白(A,B,C), 核纤层蛋白A和C是由同一个转录单位编码的, 只不过是通过可变剪接形成不同的mRNA。它们在肽链上的差别是:
-
Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry
Chemical modification reactions play an important role in various protocols for
-
Cell death detection in Xenopus embryos by ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes
-
Examination of the Axial Skeleton of Fetal Rodents
The axial skeleton represents one product of the metameric segregation of the me
-
Tissue Culture Methods
TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refer
-
MAP Kinase Regulation of the Mitotic Spindle Checkpoint
Maintaining the integrity of the cell cycle is critical for ensuring that cells
-
Derivation and Manipulation of Murine Embryonic Stem Cells
Pluripotent embryonic stem (ES) cell lines were first isolated over 25 years ago
-
Isolation and Characterization of Hepatic Stem Cells, or Oval Cells, from Rat Livers
The pace of research on the potential therapeutic uses of liver stem cells or “o
-
Biochemical Analysis of Arginine Methylation in Transcription
Protein arginine methylation has emerged as an important mechanism for regulatin
-
Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Even
The mechanisms that mediate how migratory eukaryotic cells amplify a shallow, ex
-
Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using
Quantitative mRNA characterization by reverse transcription (RT) of RNA and subs
-
Overexpression or Downregulation of mTOR in Mammalian Cells
This chapter presents an overview of the methods that have been used to overexpr
-
Control of the Interferon Response in RNAi Experiments
The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever s
-
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Genome sequences of many filamentous fungi are now available and additional geno
-
Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
The two-hybrid system (THS) (1 ) is a molecular genetic screen that detects prot
