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Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot
Mitochondrial disorders are clinically and genetically heterogeneous. There are
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Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells
Research of stem cells will enable us to understand the development and function
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应力纤维(stree fibers)
应力纤维又叫张力纤维，是真核细胞中广泛存在的一种较为稳定的束状纤维结构, 与骨骼肌中肌原纤维非常相像。应力纤维由大量平行排列的肌动蛋白组成,此外还含有其他在肌细
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Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Cell culture is an invaluable tool for investigators in numerous fields. It faci
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Conventional Micropipette-Based Patch Clamp Techniques
The patch clamp technique revolutionized the study of ion channels and is consid
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Scale-Up of Suspension and Anchorage-Dependent Animal Cells
In this chapter, scale-up is described in a laboratory context (10–20 L), but th
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On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
This chapter reviews the theory and methodological developments of on-line conce
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Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
The process of bringing a new pharmacologically active drug to market is laborio
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Measurement of Geminin Activity in Xenopus Egg Extracts
Geminin is an unstable protein that inhibits DNA replication by interfering with
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Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequore
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RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium
Knockouts by homologous recombination are frequently used to investigate the fun
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合子基因（zygotic gene）
受精卵在胚胎发育过程中表达的基因称为合子基因，受母体基因产物的激活。合子基因的表达是胚胎模式形成的开端。合子基因分为若干组，表达顺序有先有后，它们之间有着严密的
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Phylogenetic Inference and Parsimony Analysis
Application of phylogenetic inference methods to comparative endocrinology studi
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Knock-Down of Gene Expression in Hematopoietic Cells
RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-tr
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Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protei
The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins th
