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Pseudo-Immunolabelling With the AvidinBiotinPeroxidase Complex due to the Presence of Endogenous Bio
Immunodetection techniques are dependent on enzyme–protein conjugates for the vi
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Studies on Carbohydrate Xenoantigens
Naturally occurring and elicited anti-carbohydrate antibodies play a major role
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Amplification of Fungal Genomes Using Multiple Displacement Amplification
The availability of genomic DNA of sufficient quality and quantity is fundamenta
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Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture
The establishment of human embryonic stem cell (hESC) lines, as well as the rece
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Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro
Embryonic stem (ES) cells, and the inner cell mass from which they are derived,
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Automation of Fluorescent Differential Display With Digital Readout
Since its invention in 1992, differential display (DD) has become the most commo
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Determination of Nuclear Localization Signal Sequences for Krppel-Like Factor 8
Transcription factor proteins function in the nucleus to regulate gene expressio
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FISH and Immunofluorescence Staining in Chlamydomonas
Here we describe how to use fluorescence in situ hybridization and immunofluores
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Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute
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Isolation, Characterization, and Culture of Epithelial Stem Cells
It is well accepted that homeostasis of continuously renewing adult tissues, suc
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Tissue-Specific KO of ECM Proteins
The analysis of phenotypes caused by null and mutant alleles is a very powerful
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In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell
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Markers of Adult Neural Stem Cells
I provide detailed protocols for conduction and troubleshooting the key steps in
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Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protei
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Neutrophil Motility In Vivo Using Zebrafish
Zebrafish have emerged as a powerful model organism to study neutrophil chemotax
