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In Vivo Assay for Tumor Cell Invasion
We describe an in vivo invasion assay that enables the collection of invasive ce
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Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that init
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Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic An
The identification and quantitative measurements of proteins in human embryonic
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MT Spindowns from Extracts
Notes:The key variable in MT spindown experiments is ATP. Under high ATP conditi
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Retroviral Gene Transfer into Primary Human Natural Killer Cells
Modulation of intracellular signaling pathways or receptor expression in natural
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Isolation and Analysis of Lipid Rafts in CellCell Interactions
Lipid rafts are dynamic structures made up of proteins and lipids that float fre
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Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro
Currently, there is no effective treatment for photoreceptor degeneration, the m
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Blood Cell Capture on Antibody Microarrays and Monitoring of the Cell Capture Using Surface Plasmon
Blood is a tremendous source of data for diagnostic purposes. Thanks to the qual
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Coimmobilization of Enzymes and Cells
Coimmobilization of cells and/or enzymes is performed for three main reasons: fi
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The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultu
Embryoid body (EB) formation is a traditional method of inducing differentiation
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Targeted Gene Delivery by Virosomes*
In 1990, the first federally approved clinical trials for treatment of a genetic
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Classical Genetic Manipulation
A significant advantage of Pichia pastoris as an experimental system is the abil
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Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets
Megakaryocytes (MK) are hematopoietic cells present in the bone marrow that are
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in situ Hybridization Protocols for Detection of Viral DNA Using Radioactive and Nonradioactive DNA
in situ hybridization can provide accurate intracellular localization of specifi
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Assays for the Calcium Sensitivity of Desmosomes
Epithelial cells in vivo exist as confluent cell sheets, but this confluence is
