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Quantitative Determination of Collagen Crosslinks
The primary functional role of collagen is as a supporting tissue and it is now
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Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucle
Telomere maintenance has been shown to be essential for unlimited growth potenti
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Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb
The ability to produce highly purified populations of individual cell types is c
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An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation
Selection of a related or unrelated haematopoietic stem cell donor for a patient
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Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
The beta-cell ATP-sensitive potassium channel is a key component of stimulus —se
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Detection by TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannhei
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Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cel
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Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
This chapter describes the analysis of long- and short-lived plasma cells on tis
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In Situ Hybridization for Metalloproteinases and Their Inhibitors
The imminent completion of the human and mouse genome projects will reveal all m
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Profiling the Metabolic Signature of Senescence
Aging is a complex process, which involves changes in different cellular functio
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COPⅡ被膜小泡(COPⅡ coated vesicle)
由外被蛋白Ⅱ(coat protein Ⅱ, COP Ⅱ )包裹的小泡。外被蛋白是一个大的复合体，称为外被体(coatomer)。这种类型的小泡介导非选择性运输
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Isolation and Purification of Vertebrate DNAs
Isolation of DNA from surrounding tissues is a crucial initial step in any molec
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Magnetic Resonance Imaging Analysis of Embryos
Analyzing phenotypic effects in embryos produced with directed gene manipulation
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原代培养(primary culture)
原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此，较为严格地说是指成功传代之前的培养，此时的细胞保持原有细胞的基本性质，如果是正常细胞，仍然保留二
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Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
This chapter covers gene expression analysis by microarray to study and characte
