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Cloning of Hybridomas
Hybrid clones will appear within 2–3 wk after fusion (see Chapter 46 ). It is of
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The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Co
Adhesive interactions of cells with blood vessel walls under flow conditions are
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Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identifica
The nuclear envelope (NE) is a double membrane system that is both a part of the
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核酶(ribozyme)
核酶一词用于描述具有催化活性的RNA, 即化学本质是核糖核酸(RNA), 却具有酶的催化功能。核酶的作用底物可以是不同的分子, 有些作用底物就是同一RNA分子中
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细胞培养常见问题及回答
细胞培养常见问题及回答 如何选用特殊细胞系培养基？ 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，
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Generation and Establishment of Murine Adherent Cell Lines
We describe a method to derive cell lines and clones from cells of the murine mi
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Analysis of Myristoylated and Palmitoylated Src Family Proteins
Several hundred viral and cellular proteins have been shown to be covalently mod
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Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
Wnts play a central role in the development of many cells and tissue types in al
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Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies
Mammary reconstitution assays can be used to measure the stem cell frequency wit
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In Vivo and In Vitro Models of Mammalian Angiogenesis
Angiogenesis is a complex process involving the organization of proliferating en
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Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans
A strategy for determining the structure of a glycosaminoglycan (GAG) can be div
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Differentiate ES cells into glial cells and neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture
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Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA
Apoptosis is frequently triggered by events that alter the expression of key tar
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High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy
This chapter deals with tissue preparation for subsequent detection of molecules
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A Molecular Evolution Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases
Mitogen-activated protein (MAP) kinases are a large family of enzymes composed o
