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细胞冻存方法
1．细胞：选对数生长期细胞，收集细胞24小时前换液一次。2．计数：按常规方法把细胞制成细胞悬液，计数，令细胞密度达5106/ml，离心去上清，吸入离心管中。3．
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Cryopreservation of Semen from Domestic Livestock
Although the first observations concerning low temperature preservation of sperm
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Trypsinizing cells
There are many procedures with which to trypsinize cells. All include washing th
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Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny
It is now clear that the adult central nervous system contains a population of n
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Electron Cryomicroscopy of Fibrillar Collagens
Collagen fibrils in tissue are generally heterotypic with more than one type of
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Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy
Invadopodia are hair-like membrane protrusions projecting from the ventral side
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Activity-Based Profiling of Lipases in Living Cells
The ultimate goal of proteomics is to characterize the function of all proteins
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Generation of Differentially Polyglutamylated Microtubules
Microtubules are cytoskeletal structures built of alpha- and beta-tubulins. Alth
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Suppression of Pancreatic and Colon Cancer Cells by Antisense K-ras RNA Expression VectorsM. Ian Phi
Various genetic abnormalities accumulate during multistage carcinogenesis. The c
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Culture Systems: Low-Oxygen Culture
The tension of oxygen measured in the oviducts of several mammals was 5–8.7 %, b
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Peptidomics: Divide et Impera
The term “peptidomics” can be defined as the systematic analysis of the peptide
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Visualizing Receptor Endocytosis and Trafficking
G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) represent the lar
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ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells
Aldehyde dehydrogenase (ALDH) can be used as a marker to isolate, propagate, and
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Assaying Chemotaxis of Dictyostelium Cells
Both prokaryote and eukaryote cells can sense and move up chemical concentration
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卵原细胞(oogonia)
即原始生殖细胞。在人类，卵原细胞的增殖期到胚胎发育结束时已完成， 因此，女性生殖周期中能够分化成卵的所有卵原细胞在诞生时就已形成。
