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Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recom
The endocrine pancreas contains small clusters of 1,000–2,000 neuroendocrine cel
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In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
The involvement of calcium signalling during chemotaxis in Dictyostelium discoid
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Measurement of Platelet Microparticles
Platelet microparticles are submicron vesicles that can support thrombin generat
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Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification
in situ hybridization has become a powerful tool for the analysis of gene expres
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对付支原体污染的一点经验
前阵子做单抗，做了几次细胞融合都没做出来，后面怀疑是SP2/0受支原体污染，细胞生长速度减慢，出现细胞碎片，换液后情况稍好。怀疑受污染后我对细胞做了一系列的处理
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Quantitation of Methylmalonic Acid in Serum or Plasma Using Isotope Dilution-Selected Ion Gas Chroma
The measurement of plasma or serum methylmalonic acid (MMA) is useful for monito
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休克(shock)
在休克中，由于组织缺血、缺氧，影响了供能系统，造成膜的不稳定，引起溶酶体酶的外漏，造成细胞与机体的损伤。溶酶体的酶外漏的可能机理是：由于缺氧，引起细胞pH值的下
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Assays for Alloreactive Responses by PCR
In organ transplantation, major efforts are currently developed to minimize or e
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Tol2-Mediated Gene Transfer and In Ovo Electroporation of the Otic Placode: A Powerful and Versatile
The vertebrate inner ear is composed of several specialized epithelia containing
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Hormone-lnducible Genes in Prostate Cells
Prostate gland development, growth, function and pathology are strongly influenc
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Multiple Linear Regression
This chapter describes multiple linear regression, a statistical approach used t
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Retroviral Transduction of Murine Hematopoietic Stem Cells
Hematopoietic stem cells (HSC) are inherently rare cell types that cannot be obt
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In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells
Efficient derivation and isolation of hematopoietic stem cells (HSCs) from human
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全血细胞培养染色体技术常用试剂配制
一、国内实验室应用试剂配制 （一）培养液 （1）RPMI1640培养液：称取RPMI培养基9.8g，碳酸氢钠1.9g、青霉素100u/ml，加双蒸水至1000m
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EGF受体(EGF receptor)
EGF受体是一种糖蛋白, 广泛分布于哺乳动物的上皮细胞、人的成纤维细胞、胶质细胞、角质细胞等。EGF 受体是一条含有1186个氨基酸残基的多肽链, 相对分子质量
