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Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentia
We document the protocols and methods for the production of immortalized cell li
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Generation and Detection of S-Nitrosothiols
Nitric oxide (NO) plays a pivotal role in cellular signaling in many different o
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Endothelial and Cardiac Regeneration from Adipose Tissues
For a long time, adipose tissue was only considered for its crucial role in ener
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Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos
Radiolabeled compounds that are substrates for transmembrane transporters can be
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Methods for Detection of ProteinProteinnl and ProteinDNA Interactions Using HaloTag
HaloTag™ is a protein fusion tag which was genetically engineered to covalently
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Convergent Extension Analysis in Mouse Whole Embryo Culture
Mutations have been identified in a non-canonical Wnt signalling cascade (the pl
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Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
The gastrointestinal tract of vertebrates is lined by epithelium that develops f
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Thymic Transplantation in Pig-to-Nonhuman Primates for the Induction of Tolerance Across Xenogeneic
With the advent of knockout pigs for α1,3-galactosyltransferease (GalT-KO, which
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Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter
Recombinant protein expression in heterologous hosts such as Escherichia coli (E
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SAPK/Jun kinase assays
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS
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Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human
The human placenta is a unique and still very poorly understood organ During ges
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Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells
Although embryonic stem (ES) cells have the regenerative capability of producing
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Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosectio
MicroRNAs (miRNAs) are 20−25 nucleotide long, noncoding, and single-strand RNAs
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Isolation and Characterization of Mouse Side Population Cells
The side population (SP) is a subpopulation of mouse bone marrow cells highly en
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肿瘤发生(tumorigenesis)
正常细胞发展成为肿瘤的过程称为肿瘤发生。从细胞水平上看，癌的发生是极偶然的事件。从遗传上看，癌都是由一个细胞发展而来，由一个失去了增殖控制的细胞发展而来。人体有
