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In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation
Electroporation generally refers to the technique of permeabilizing cell membran
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Introduction: Why Analyze Single Cells
Powerful methods in molecular biology are abundant; however, in many fields incl
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Methods for Homologous Recombination in Drosophila
We present detailed protocols for two methods of gene targeting in Drosophila .
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Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo
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Subtelomeric and/or Subcentromeric Probe Sets
Aside from microscopically visible chromosomal rearrangements, numerous cryptic
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Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for th
This chapter is a step-by-step protocol for setting up, realizing, and analyzing
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小鼠重组胰岛素抗体酶联免疫分析（ELISA）
小鼠重组胰岛素抗体 酶联免疫 分析（ ELISA ）试剂 盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中
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Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides
The Xenopus laevis oocyte offers one of the most convenient expression systems f
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Recycling Tubulin
Recycling Tubulin We "recycle" tubulin fractions stored at -80¡C after
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Overview: Imaging in the Study of Integrins
Integrins play critical adhesion and signaling roles during development, wound h
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Use of Disulfide Cationic Lipids in Plasmid DNA Delivery
Gene therapy provides a paradigm of the treatment of human diseases. The ultimat
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Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin
Colloidal gold probes, including protein A-, IgG-F(ab’ )2 -, and streptavidin-la
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Using Drosophila Larval Imaginal Discs to Study Low-Dose Radiation-Induced Cell Cycle Arrest
Under genotoxic stress, activation of cell cycle checkpoint responses leads to c
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Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis
Nitric oxide (NO) is an important signaling molecule that plays a key role in va
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Capillary Electrophoresis of Gene Mutation
This chapter illustrates the usefulness of capillary electrophoresis (CE) for th
