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Engineered Properties and Assays for Human DNase I Mutants
Human deoxyribonuclease (DNase I) is an important clinical agent currently used
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Current Status of the Polyamine Research Field
This chapter provides an overview of the polyamine field and introduces the 32 o
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Electroporation of Muscle Tissue In Vivo
Electroporation (also termed electropermeabilization) of muscle tissue has been
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Amphibian Organizer Activity
The amphibian organizer corresponding to the upper dorsal blastopore lip of the
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Cell Senescence Culturing Methods
Development of therapeutic approaches that slow or ablate the adverse physiologi
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Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
The events that determine whether hematopoietic stem cells (HSC) divide in the c
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Mitophagy and Mitoptosis in Disease Processes
Mitochondria play a very important role in cellular function, not only through k
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Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks
Generation of double-strand breaks (DSBs) in chromosomal DNA induces repair mach
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Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells
The last decade has seen major advances in our knowledge of the molecular contro
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Functional Analysis by Inducible RNA Interference in Drosophila melanogaster
Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become
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我对付支原体污染的一点经验
前阵子做单抗,做了几次细胞融合都没做出来,后面怀疑是SP2/0受支原体污染,细胞生长速度减慢,出现细胞碎片,换液后情况稍好。怀疑受污染后我对细胞做了一系列的处理
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In Vivo Labeling and Analysis of Epidermal Stem Cells
To identify slowly cycling (label-retaining) keratinocytes in the cutaneous epit
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Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domai
Structural data are critical for an understanding of how membrane transport syst
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Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage
The targeted delivery of fluorescently labeled, DNA-modifying proteins into cell
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Scanning Electron Microscopy of Bone
This chapter described methods for Scanning Electron Microscopical imaging of bo