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Routine Culturing of ES Cells
Cell are normally passaged every 2-3 days, this is important to avoid differenti
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Quantitative Determination of Collagen Cross-links
The primary functional role of collagen is as a supporting tissue and it is now
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形态发生素(morphogen)
携带决定细胞分化方向相关信息的可扩散的物质。形态发生素是以连续的浓度梯度分布的，因而形成各种浓度阈值，细胞根据所处环境的形态发生素的浓度阈值决定分化方向。形态发
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Transplantation Chimeras: Use in Analyzing Mechanisms of Avian Development
The word chimera originally referred to a mythological beast with the head of a
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Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2
Telomerase, a ribonucleoprotein enzyme, is detected in the vast majority of canc
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In Vitro and In Vivo Assays of B-Lymphocyte Migration
The development and functional activities of B lymphocytes critically depend on
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Lipid Raft-Redox Signaling Platforms in Plasma Membrane
Membrane lipid rafts (LRs) have been demonstrated to be importantly involved in
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Mouse ES Cell-Derived Hematopoietic Progenitor Cells
Future stem cell-based therapies will benefit from the new discoveries being mad
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Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells
Conventionally, human embryonic stem cells (hESCs) are cultured on feeder cells.
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Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microsco
A number of techniques have been developed to use chemical, immunological, and m
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桥粒(desmosomes)
在斑块连接中，如果细胞是通过中间纤维锚定到细胞骨架上，这种粘着连接方式就称为桥粒。桥粒连接也分为两种情况:如果涉及的是相邻两细胞间的连接，则称为桥粒 (实际上是
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Classes and Prediction of Cell-Penetrating Peptides
The classical view on how peptides enter cells has been changed due to the devel
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Extraction of Nucleic Acids from Bone
Here, we present methods for extracting DNA and RNA from samples of whole bone t
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Scale-Up of Single CellInoculated Suspension Cultures of Human Embryonic Stem Cells
We have developed a simple yet highly reproducible technique of upscaling single
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Preparation of Chick Striated Muscle Cultures
The maturation of striated muscle in primary cultures closely parallels the form
