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Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells
Quantification of the expression of individual genes can reveal much concerning
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Drosophila Cell Lines as Model Systems and as an Experimental Tool
Given the power of Drosophila genetics, it may seem surprising to discover that
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Quantitation of Transient Gene Expression
In many situations, it is important to quantitate accurately expression of a tra
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Basics of Flow Cytometry
Flow cytometry is a laser-based technology that is used to measure characteristi
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Quantitative Estimation of Tissue Blood Flow Rate
Tissue blood flow rate (F ) is a critical parameter for assessing functional eff
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Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin
Langerhans cells (LCs) are the main population of antigen-presenting cells linin
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Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monola
Increased vascular permeability is the hallmark of inflammation. Here, we descri
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Cloning of the 3 Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Diffe
Several structural and metabolically important proteins in eukaryotes are encode
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Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryo
Human embryonic stem cells (hESCs) are an invaluable cell source to study human
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Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients
Septic syndromes represent a major, although largely under-recognized, healthcar
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Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
The development of primordial germ cells into oocytes within primordial follicle
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Strategies to Insulate Lentiviral Vector-Expressed Transgenes
Lentiviruses are capable of infecting many cells irrespective of their cycling s
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Detection of Telomerase Activity in Neural Cells
Telomeres are specialized nucleoprotein structures that cap linear eukaryotic ch
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Assay for the Micrococcal Nuclease
Method: Essentially that described by Heins et al. (1966) based upon the release
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Enrichment of Human Platelet Membranes for Proteomic Analysis
Platelets (thrombocytes) are the smallest human blood cells and are pivotal in p
