One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and

The procedure described in this chapter is used to display single-stranded nucleic acids according to their sizes, within the range of 0.5 to 30 kilobases (kb). Possible applications include examining products of in vitro synthesis, hybrid-selected RNAs from total cellular nucleic acids, and Northern blots. The method works equally well with RNA and DNA.