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Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads
Immunomagnetic beads are uniform, polymer particles coated with a polystyrene sh
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SPARK: A New Peptidyl Transferase Activity Assay
The formation of peptide bonds is the central chemical reaction during protein s
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Spectrophotometric Methods for the Determination of Superantigen Structure and Stability
An important characteristic of superantigens (SAgs) is their high structural sta
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Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
Camelid single domain antibodies fused to noncamelid Fc regions, also called chi
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Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
A major obstacle in studying monocyte cell biology has been the resistance of th
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Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression
MicroRNAs (miRNAs) constitute a class of molecules regulating gene expression in
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Staining Protocol for Superantigen-Induced Cytokine Production Studied at the Single-Cell Level
In order to get the full picture of the cytokine response generated by stimulati
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Staining for total receptors
Staining for total receptorscontributed by Patricia TsaoThis protocol will visua
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STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Mat
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Standardization and Validation Issues of the ELISPOT Assay
During the last 20 yr, the enzyme-linked immunospot (ELISPOT) assay has emerged
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Static Energy Analysis of MHC Class I and Class II Peptide-Binding Affinity
Antigenic peptide is presented to a T-cell receptor (TCR) through the formation
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Statistical Analysis of ELISPOT Assays
Cytokine ELISPOT assays have emerged as a powerful tool for the detection of rar
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Stem Cell Transplantation: Graft-Mediated Antileukemia Effects
Graft-mediated antileukemia (GVL) activity is a major factor contributing to the
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Stimulation of Mucosal Immunity
The mucosal immune system is composed of distinct regional immune tissue (e.g.,
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Strategies for the Isolation and Characterization of Antibacterial Lantibiotics
Lantibiotics are biologically active peptides produced by several strains from t