In Vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates

Sterile particulates such as monosodium urate crystals induce inflammasome activation resulting in activation of caspase-1, secretion of IL-1α, and processing of IL-1β. Local production and activation of IL-1 leads to neutrophil recruitment in vivo. Here we describe two quick and simple methods for the evaluation of neutrophil recruitment in the peritoneal cavity and skin in response to sterile particulates, which are dependent on IL-1 receptor signaling.