Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA

Hyperphage is a substitute for M13KO7 helper phage used in antibody phage display. It allows to improve the antibody display efficiency on phage by 2-3 orders of magnitude. This is achieved by forcing the packaging E. coli cell to exclusively use the pIII portion of the antibody fusion protein for its virion assembly. Hyperphage is compatible with all usual phagemid systems using a full length pIII gene in fusion to the binder repertoire, and has been widely validated to improve panning with antibody and scaffold libraries.