Gene Therapy With Immunotoxins

The purpose of this chapter is to describe the method of treating lymphokine-activated killer (LAK) cells with retroviral immunotoxins (retIT) so that these cells can express and secrete immunotoxins (IT). The intent is to use LAK as a vehicle to deliver IT therapy directly to leukemia cells in vivo. This would reduce their systemic toxicity and thus solve a major problem that has limited the use of IT clinically.