Water Treatment with DEPC
 Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 
        | Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC by causing hydrolysis of DEPC. | 
| Notes: DEPC is highly toxic. | 
 
  
           
              
