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什么是免疫荧光技术？
免疫荧光技术是以荧光物质标记抗体而进行抗原定位的技术。用荧光抗体示踪或检查相应抗原的方法称荧光抗体法；用已知的荧光抗原标记物示踪或检查相应抗体的方法称荧光抗原法
2025-02-11 10 -
细胞/组织免疫荧光实验操作步骤
以某细胞样本为例：一、准备免疫荧光所需的仪器与试剂：CO2恒温培养箱、倒置荧光显微镜、离心机、超净工作台、DMEM培养基、胎牛血清、胰酶、抗生素二、细胞免疫荧光
2025-02-11 9 -
免疫荧光实操注意事项汇总
那么免疫荧光有哪些注意事项呢？1、组织分切后，应迅速置入冰壶，并立即送至恒温切片机内进行包埋切片。2、如果不能马上切片，应将标本立即置入零下七十度的低温冰箱内以
2025-02-11 11 -
免疫荧光常见问题及其解决方法
免疫荧光常见问题一：目标蛋白的荧光很弱，导致组间差异并不明显，造成假阴性结果这种情况出现后，首先要检查一下抗体对不对，是否适用于你的预处理组织。有些抗体只适用于
2025-02-11 6 -
什么是细胞增殖？
细胞增殖是生物体的重要生命特征，细胞以分裂的方式进行增殖。单细胞生物，以细胞分裂的方式产生新的个体。多细胞生物，以细胞分裂的方式产生新的细胞，用来补充体内衰老或
2025-02-11 6 -
细胞增殖检测方法汇总
细胞增殖检测法（一）：直接计数法这是一种非常简单的检测方法，我们可以利用计数板或技术仪得出细胞数目然后对数目进行比较即可。其缺点是不适合评估特定亚群、和数量较多
2025-02-11 6 -
Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Nativ
Voltage-gated K+ channels (KV channels) are encoded by the KCNx gene family and
2025-02-11 7 -
Isolation and Characterization of Cancer Stem Cells In Vitro
The cancer stem cell hypothesis is an appealing concept to account for intratumo
2025-02-11 6 -
Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyr
Analysis of the human genome sequence has identified thousands of putative genes
2025-02-11 4 -
Mosaic Analysis in Caenorhabditis elegans
The complete description of its nearly invariant cell lineage (1 ,2 ) and the gr
2025-02-11 6 -
Fine Structure of the Autophagosome
This chapter describes the electron microscopic fine structure of early and late
2025-02-11 4 -
Antisense Therapeutics in the Central Nervous System: The Induction of c-fos
Immediate-early genes (IEGs) are members of a class of genes that respond, in ma
2025-02-11 5 -
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
We present a method that uses microcontact printing of alkanethiols on gold to g
2025-02-11 4 -
Determination of Gene Promoter Activity in Skeletal Muscles In Vivo
The use of nonviral (plasmid DNA) gene delivery into skeletal muscle has increas
2025-02-11 5 -
Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
Microscopic imaging of single fluorescent molecules within cells provides a mole
2025-02-11 5
