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细胞凋亡检测的各个阶段的对应方案
相关专题 细胞凋亡概念的提出虽然不过40年,却由于其在生命个体和细胞中的重要作用成为当今科学界的热门话题,现在已有不少针对细胞不同周期的细胞凋亡检测 方法,本
2025-02-26 10 -
Hela细胞传代培养的操作步骤
相关专题 1.观察培养基是否正常,细胞状态如何,细胞密度如何,决定是否传代。观察时拧紧盖子。2.加热PBS 、versene、培养基,(提前做好) 瓶子不要倒
2025-02-26 10 -
电化学梯度(electrochemical gradient)
质子跨过内膜向膜间隙的转运也是一个生电作用(electrogenesis),即电压生成的过程。因为质子跨膜转运使得膜间隙积累了大量的质子,建立了质子梯度。由于膜
2025-02-26 10 -
内质网滞留信号(ER retention signal)
内质网的结构和功能蛋白羧基端的一个四肽序列: Lys-Asp-Glu-Leu-COO-,即KDEL信号序列。这段序列在高尔基体的膜受有相应的受体, 一旦进入高尔
2025-02-26 19 -
TOP10 chemically competent cells
OverviewThis protocol is a variant of the Hanahan protocol [1] using CCMB80 buff
2025-02-26 10 -
Extraction of Chromatin
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise
2025-02-26 9 -
人白细胞介素5(IL-5)酶联免疫分析(ELISA)
人 白细胞介素5(IL-5) 酶联免疫 分析(ELISA)试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样
2025-02-26 13 -
Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both
2025-02-26 9 -
Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dyna
Mitotic spindle microtubules pull chromosomes toward each pole to generate two d
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Fluorescence Recovery After Photobleaching
This chapter describes the use of microscope-based fluorescence recovery after p
2025-02-26 11 -
Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
Mesenchymal stem cells also called mesenchymal stromal cells (MSCs) are multipot
2025-02-26 11 -
Simultaneous analysis of DNA content and surface immunophenotyp
Simultaneous analysis of DNA content and surface immunophenotype using gentle et
2025-02-26 14 -
UVB致皮肤角质形成细胞的损伤模型的制作方法
材料和方法 1 试剂:胎牛血清(北京军医兽医防治中心) ,DMEM培养液(GIBCO ,美国) ,碘化丙啶PI(Sigma) ,Triton2X100 (BDH
2025-02-26 8 -
兔成骨细胞与骨髓基质干细胞混合培养的实验研究
对骨髓基质干细胞进行改造,使其在保持快速生长的同时向成骨细胞方向分化是目前国内外研究的热点。实验发现,成熟的成骨细胞可以通过细胞间的直接接触作用促进骨髓基质干细
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Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Ner
2025-02-26 8