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Purification Through Affinity Chromatography and Microsequencing
Production of monoclonal antibodies (MAbs) using adhesion-involved cell types as
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Local Application of Antisense for Prevention of Restenosis
In 1977, percutaneous transluminal coronary angioplasty (PTCA) was first introdu
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小泡运输(transport by vesicles)
蛋白质从内质网转运到高尔基体以及从高尔基体转运到溶酶体、分泌泡、细胞质膜、细胞外等则是由小泡介导的,这种小泡称为运输小泡(transport vesicles)
2025-02-25 14 -
前中期(prometaphase)
介于前期与中期之间的一个过渡期。前中期的主要特征是染色体剧烈地活动, 个别染色体剧烈地旋转、振荡、徘徊于两极之间。此时期的主要事件是纺锤体(spindle)的装
2025-02-25 10 -
The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemilumine
A plethora of publications on techniques and methodologies for measuring nitric
2025-02-25 8 -
Protein Degradation Via the Proteosome
It is becoming apparent that an increase in the rate of protein degradation repr
2025-02-25 14 -
Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
The nematode Caenorhabditis elegans is an excellent model organism for studying
2025-02-25 10 -
Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissu
With the use of lasers for ablation purposes in spinal surgery, the tissue tempe
2025-02-25 11 -
Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols
2025-02-25 10 -
Assays of MSCs with Microarrays
The rapid development of microarray technology during the last decade has greatl
2025-02-25 10 -
Oligonucleotide FISH Probes
The use of highly specific synthetic oligonucleotide (ODN) probes, which have su
2025-02-25 14 -
Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional An
In this chapter, we explain different strategies to analyze the extracellular He
2025-02-25 14 -
动物细胞大规模微载体培养技术
相关专题 一、微载体 培养应用 此技术于1967年被用于动物细胞大规模培养。经过三十余年的发展,该技术目前已渐日趋完善和成熟,并广泛应用于生产疫苗、基因工程产
2025-02-25 14 -
Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped pla
2025-02-25 10 -
The Analysis of tRNA Import Into Mammalian Mitochondria
Ribonucleic acid (RNA) import into mitochondria occurs in a variety of organisms
2025-02-25 8