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RNA Extraction From Mammalian Tissues
Purification of intact RNA is the primary step of many molecular biology techniq
2025-02-24 13 -
PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
RNA localization is an important cellular mechanism for restricting the activiti
2025-02-24 14 -
Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
The use of tissue-selective rather than ubiquitous knockouts of steroid receptor
2025-02-24 15 -
绒毛染色体制备方法
绒毛来源于胚胎的中胚层，最早的初级干绒毛出现于孕三周初，孕8周左右是绒毛发育最旺盛时期。目前国内外绒毛取材多选于8-9周。研究资料表明，绒毛取样不影响胚胎的发育
2025-02-24 17 -
FISH on Plant Chromosomes
FISH, which allows the mapping of DNA sequences directly onto chromosomes, has r
2025-02-24 11 -
Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
The following protocols provide a rapid approach for establishing good working c
2025-02-24 11 -
Computational Reconstruction of Ancestral DNA Sequences
This chapter introduces the problem of ancestral sequence reconstruction: given
2025-02-24 10 -
Characterization of Genes for Polyamine Modulon
Polyamines are essential for normal cell growth and exist mainly as RNA-polyamin
2025-02-24 11 -
Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture
Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs
2025-02-24 17 -
Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Continuous nucleocytoplasmic shuttling of signal transducer and activator of tra
2025-02-24 11 -
Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have been used to derive self-renewing neur
2025-02-24 8 -
T Cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T Cells in Chronic Inte
Inflammatory bowel diseases (IBD) consist of Crohn’s disease (CD) and ulcerative
2025-02-24 9 -
Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
The metabolic process of glycosylation of proteins on the amide nitrogen of spec
2025-02-24 12 -
Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
The hematopoietic stem cells (HSCs) constitute an ideal target for the gene ther
2025-02-24 11 -
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
This chapter provides basic information for quantifying soluble epidermal growth
2025-02-24 11
