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双荧光素酶实验细胞一般转染多久?
双荧光素酶报告基因实验细胞转染时间一般为48小时。双荧光素酶报告基因用于实验系统中作相关的或成比例的检测,通常一个报告基因作为内对照,使另一个报告基因的检测均一
2025-02-07 6 -
免疫荧光染不上色怎么办?
免疫荧光技术是标记免疫技术中发展最早的一种.它是在免疫学、生物化学和显微镜技术的基础上建立起来的一项技术。那么免疫荧光染不上色的原因和处理方法有哪些?我们来一个
2025-02-07 10 -
彗星实验结果怎么分析?
答:彗星实验又称单细胞凝胶电泳实验,是一种常用的可以快速检测单细胞DNA损伤程度的方法,彗星实验的结果有阴性和阳性两种,分析对比图如下:阴性对照没有拖尾的形状而
2025-02-07 9 -
双荧光素酶的两个报告基因载体作共转染应用什么条件呢?
当用双荧光素酶报告基因测试系统来测量等重量的萤火虫荧光素酶及海肾荧光素酶时,可观察到它们释放的光几乎相等。萤火虫荧光素酶的分子虽为 61kD ,而海肾荧光素酶的
2025-02-07 5 -
裸鼠成瘤肿瘤为什么长起来又消失了?
裸鼠成瘤实验是将体外培养的肿瘤细胞接种到有免疫缺陷的裸鼠身上,促进肿瘤的形成,用来研究和测试肿瘤和抗肿瘤药物。但在造模过程中我们经常遇到长出来的瘤子过一段时间又
2025-02-07 6 -
High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
A high-throughput approach to prokaryotic differential display has been develope
2025-02-07 9 -
CDK-Activating Kinases: Detection and Activity Measurements
All cyclin-dependent kinases (CDKs) involved in eukaryotic cell cycle control re
2025-02-07 8 -
Visualization of mRNA Localization in Xenopus Oocytes
Visualization of in vivo mRNA localization provides a tool for understanding ste
2025-02-07 9 -
常规组织初代消化培养
相关专题 1. 准备:取各种已消毒的培养用品置于净化台面,紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。2. 布局:点燃酒精灯,安装吸管帽。3
2025-02-07 9 -
The Human Embryo: Ethical and Legal Aspects
This paper analyses the status of the embryo in Canadian law. First, a brief ove
2025-02-07 10 -
MT Spindowns from Extracts
MT Spindowns from Extracts Arshad Desai Notes:The key variable in MT spindown ex
2025-02-07 15 -
Identification and Quantitation of G-Protein α-Subunits
The demonstration that many intracellular signaling processes are mediated by a
2025-02-07 7 -
In Vivo Measurement of Glioma-Induced Vascular Permeability
The normal blood–brain barrier (BBB) consists of tight interendothelial cell jun
2025-02-07 9 -
细胞培养常见问题、可能原因及解决方法
问题1:培养液pH 值变化太快 可能原因 (1)CO2 张力不对 (2)培养瓶盖拧得太紧 (3)NaHCO3 缓冲系统缓冲力不足 (4)培养液中盐浓度不正确 (
2025-02-07 10 -
Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extre
Unusual long polyamines such as caldopentamine and caldohexamine, and branched p
2025-02-07 21