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Site-Specific Cleavage of Fusion Proteins
Where an affinity tag has served its purpose, it may become desirable to remove
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Preparation of Low Density, Collagenase-Digested Splenocytes (Hedrick Lab, UCSD Cancer Center)
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/M
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A Practical Guide for using the AdEasy System
相关专题Tong-Chuan He, Kenneth W. Kinzler, and Bert Vogelstein Howard Hughes Medical
2025-02-22 9 -
Transfection of Osteoclasts and Osteoclast Precursors
Osteoclasts and their precursors have traditionally been considered difficult ce
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Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
The kidney is an extremely heterogeneous organ from a morphological and a functi
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Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
urpose: Cell lines are reactivated and grown to a count of 1 x 108 cells. The c
2025-02-22 10 -
Site-Directed Mutagenesis in the Study of Membrane Transporters
One of the major goals in membrane transporter research is to understand how tra
2025-02-22 9 -
Analysis of Proteins from Marine Molluscs
Application of the two-dimensional gel electrophoresis (2DE) protocols which wer
2025-02-22 9 -
The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma
Past literature on exposure to lipophilic agents such as organochlorines (OCs) i
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The Use of Calorie Restriction Mimetics to Study Aging
Calorie restriction (CR) has a variety of effects on extending lifespan and dela
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Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures
The study of glial cell development and function has been considerably enhanced
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MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TI
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Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
Cryofixation and freeze-substitution techniques preserve plant ultrastructure mu
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PKA系统(protein kinase A system, PKA)
是G蛋白偶联系统的一种信号转导途径。信号分子作用于膜受体后，通过G蛋白激活腺苷酸环化酶, 产生第二信使cAMP后,激活蛋白激酶A进行信号的放大。故将此途径称为P
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Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
Several investigations, carried out in either artificial or cellular models and
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