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  • Assay for Protein Modification by Poly-ADP-Ribose In Vitro

    Assay for Protein Modification by Poly-ADP-Ribose In Vitro

    The enzymatic function of poly(adenosine diphosphate (ADP)-ribose) polymerase (P

    2025-02-22 20
  • Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp

    Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp

    Over the last nine years, several quantitative polymerase chain reaction (QPCR)

    2025-02-22 17
  • Expression of EGF and HIV Envelope Glycoprotein

    Expression of EGF and HIV Envelope Glycoprotein

    This chapter reviews the expression of two proteins that are naturally secreted:

    2025-02-22 18
  • Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

    Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

    Progression of cells through successive phases and checkpoints of the cell cycle

    2025-02-22 15
  • Extrachromosomal Assay for DNA Double-Strand Break Repair

    Extrachromosomal Assay for DNA Double-Strand Break Repair

    DNA double-strand break (DSB) repair in mammalian cells has been demonstrated to

    2025-02-22 16
  • Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv

    Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv

    In order to restore or to introduce a gene function integrating viral vector sys

    2025-02-22 17
  • Direct Sequencing of PCR Products Using Chemiluminescent Detection

    Direct Sequencing of PCR Products Using Chemiluminescent Detection

    The following protocol describes a method that can be used for the direct sequen

    2025-02-22 18
  • Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals

    Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals

    T-cell motility is critical for leukocyte trafficking both in normal host defens

    2025-02-22 11
  • Computer-Assisted Interpretation of Triacylglycerols Mass Spectra

    Computer-Assisted Interpretation of Triacylglycerols Mass Spectra

    Triacylglycerols (TGs) are principal components of vegetable oils and animal fat

    2025-02-22 13
  • 胶原(collagen)

    胶原(collagen)

    胶原是细胞外最重要的水不溶性纤维蛋白, 是构成细胞外基质的骨架。胶原在细胞外基质中形成半晶体的纤维,给细胞提供抗张力和弹性,并在细胞的迁移和发育中起作用。胶原在

    2025-02-21 24
  • Group Migration and Signal Relay in Dictyostelium

    Group Migration and Signal Relay in Dictyostelium

    The ability of cells to migrate directionally in gradients of chemoattractant is

    2025-02-21 20
  • Purifying the Hedgehog Protein and its Variants

    Purifying the Hedgehog Protein and its Variants

    The purification of recombinant versions of the N-terminal signaling fragment of

    2025-02-21 19
  • Quantitative Visualization of Autophagy Induction by mTOR Inhibitors

    Quantitative Visualization of Autophagy Induction by mTOR Inhibitors

    Autophagy is a catabolic pathway that degrades bulk cytosol in lysosomal compart

    2025-02-21 14
  • Studying Early Hematopoiesis Using Avian Blastoderm Cultures

    Studying Early Hematopoiesis Using Avian Blastoderm Cultures

    The process of hematopoiesis occurs very early during embryogenesis, with the ap

    2025-02-21 17
  • 125I-UdR致C6胶质瘤细胞损伤的模型

    125I-UdR致C6胶质瘤细胞损伤的模型

    一、材料:125I-UdR购自中科院北京原子能所,放化纯度>95%。脱氧腺苷(AdR)、脱氧鸟苷(GdR)、脱氧胞苷(CdR)、脱氧尿苷(UdR)均为Si

    2025-02-21 17