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  • Ras GTPases Are Both Regulators and Effectors of Redox Agents

    Ras GTPases Are Both Regulators and Effectors of Redox Agents

    Redox agents have been historically considered pathological agents which can rea

    2025-02-06 6
  • Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies

    Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies

    Sea urchins have long been used to study morphogenesis and cell fate specificati

    2025-02-06 6
  • 停靠蛋白(docking protein, DP)

    停靠蛋白(docking protein, DP)

    即SRP在内质网膜上的受体蛋白,它能够与结合有信号序列的SRP牢牢地结合,使正在合成蛋白质的核糖体停靠到内质网上来。停靠蛋白含有两个亚基,一个亚基暴露于细胞质的

    2025-02-06 7
  • Laboratory Guidelines for Animal Care

    Laboratory Guidelines for Animal Care

    Animal research is a controversial subject because of the ethical and moral impl

    2025-02-06 5
  • Differentiate ES cells into cystic embryoid bodies

    Differentiate ES cells into cystic embryoid bodies

    Differentiate ES cells into cystic embryoid bodiesDay -1: Pass ES cells at norma

    2025-02-06 7
  • Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo

    Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo

    The use of ionizing radiation as a therapeutic agent has been recognized for alm

    2025-02-06 8
  • Apoptosis: Miniassay

    Apoptosis: Miniassay

    1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3)

    2025-02-06 5
  • Senescence Regulation by the p53 Protein Family

    Senescence Regulation by the p53 Protein Family

    p53, a guardian of the genome, exerts its tumor suppression activity by regulati

    2025-02-06 8
  • Molecular Profiling of the Epidermis: A Proteomics Approach

    Molecular Profiling of the Epidermis: A Proteomics Approach

    Mouse skin has been used extensively as a model system to study the development

    2025-02-06 7
  • Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies

    Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies

    The advent of the “GFP technology” together with advances in digital imaging and

    2025-02-06 5
  • Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells

    Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells

    Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated

    2025-02-06 5
  • Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells

    Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells

    The integrity, or barrier function, of the intestinal epithelium is of paramount

    2025-02-06 5
  • Screening for Inhibitors of Microtubule-Associated Motor Proteins

    Screening for Inhibitors of Microtubule-Associated Motor Proteins

    The mitotic spindle is an important target for cancer chemotherapy. The main pro

    2025-02-06 6
  • Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis

    Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis

    The hallmark of hematopoietic stem and progenitor cells (HSPCs) is their motilit

    2025-02-06 5
  • 球体细胞培养

    球体细胞培养

    球体细胞培养 1. 琼脂铺底的培养瓶:30ml无菌培养瓶,每瓶中加5 ml 2%琼脂培养基,冷却,形成平坦底层后备用。 2.取生长状态良好已连接成片的细胞,用弯

    2025-02-06 10