-
Ras GTPases Are Both Regulators and Effectors of Redox Agents
Redox agents have been historically considered pathological agents which can rea
2025-02-06 6 -
Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
Sea urchins have long been used to study morphogenesis and cell fate specificati
2025-02-06 6 -
停靠蛋白(docking protein, DP)
即SRP在内质网膜上的受体蛋白,它能够与结合有信号序列的SRP牢牢地结合,使正在合成蛋白质的核糖体停靠到内质网上来。停靠蛋白含有两个亚基,一个亚基暴露于细胞质的
2025-02-06 7 -
Laboratory Guidelines for Animal Care
Animal research is a controversial subject because of the ethical and moral impl
2025-02-06 5 -
Differentiate ES cells into cystic embryoid bodies
Differentiate ES cells into cystic embryoid bodiesDay -1: Pass ES cells at norma
2025-02-06 7 -
Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
The use of ionizing radiation as a therapeutic agent has been recognized for alm
2025-02-06 8 -
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3)
2025-02-06 5 -
Senescence Regulation by the p53 Protein Family
p53, a guardian of the genome, exerts its tumor suppression activity by regulati
2025-02-06 8 -
Molecular Profiling of the Epidermis: A Proteomics Approach
Mouse skin has been used extensively as a model system to study the development
2025-02-06 7 -
Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
The advent of the “GFP technology” together with advances in digital imaging and
2025-02-06 5 -
Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated
2025-02-06 5 -
Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells
The integrity, or barrier function, of the intestinal epithelium is of paramount
2025-02-06 5 -
Screening for Inhibitors of Microtubule-Associated Motor Proteins
The mitotic spindle is an important target for cancer chemotherapy. The main pro
2025-02-06 6 -
Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis
The hallmark of hematopoietic stem and progenitor cells (HSPCs) is their motilit
2025-02-06 5 -
球体细胞培养
球体细胞培养 1. 琼脂铺底的培养瓶:30ml无菌培养瓶,每瓶中加5 ml 2%琼脂培养基,冷却,形成平坦底层后备用。 2.取生长状态良好已连接成片的细胞,用弯
2025-02-06 10