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  • Electron Microscopic Studies of Receptor Localization

    Electron Microscopic Studies of Receptor Localization

    The localization and density of G-protein-coupled receptors (GPCRs) on the cell

    2025-01-24 6
  • Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters

    Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters

    An important approach in studies of normal, diseased, and malignant cells is the

    2025-01-24 4
  • Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillar

    Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillar

    Within the last few years, capillary electrophoresis (CE), especially with indir

    2025-01-24 7
  • Mouse Tissue Culture Models of Unstable Triplet Repeats

    Mouse Tissue Culture Models of Unstable Triplet Repeats

    Once into the expanded disease-associated range, trinucleotide repeat alleles be

    2025-01-24 8
  • Thin-Layer Chromatography of Phospholipids

    Thin-Layer Chromatography of Phospholipids

    Thin-layer chromatography (TLC) is a technique that has been routinely used for

    2025-01-24 6
  • Fluorescence In Situ Hybridization on Single Cells: Sex Determination and Chromosome Rearrangements

    Fluorescence In Situ Hybridization on Single Cells: Sex Determination and Chromosome Rearrangements

    Fluorescence in situ hybridization (FISH) is the technique of choice for preimpl

    2025-01-24 7
  • Release Testing of Retroviral Vectors and Gene-Modified Cells

    Release Testing of Retroviral Vectors and Gene-Modified Cells

    This chapter will review the design and execution of release testing requirement

    2025-01-24 5
  • A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a

    A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a

    Cleanup technology and mass spectrometric determination of sphingosine-1-phospha

    2025-01-24 7
  • 裸鼠成瘤肿瘤长不出来怎么办?

    裸鼠成瘤肿瘤长不出来怎么办?

    答:1、肿瘤生长周期很慢,一周开始生长肿块,大约需要1~2个月才能长成肿瘤。 2、细胞状态差,活力不好,或是肿瘤细胞不易成瘤,需加入

    2025-01-23 5
  • 做免疫组化时脱片怎么办?

    做免疫组化时脱片怎么办?

    答:1、多聚赖氨酸玻片质量的问题,购买切片时要注意品牌质量。 2、组织切的不好,切片机的问题例如比较老的旧的机器切的厚或者不均匀,

    2025-01-23 7
  • 免疫荧光结果背景强怎么办?

    免疫荧光结果背景强怎么办?

    答:免疫荧光背景强是指不能清晰的看到实验目的中的特异性染色,有其他荧光的干扰,影响染色结果的分析和判断。出现荧光背景强的原因和处理如下:

    2025-01-23 12
  • 巨噬细胞纯化的方法有哪些?

    巨噬细胞纯化的方法有哪些?

    1、贴壁法纯化巨噬细胞 (1)用含20%~40%小牛血清的RPMI-1640培养液将巨噬细胞配成2106~4106/ml的浓度。以

    2025-01-23 8
  • 流式分选和磁珠分选有什么区别?

    流式分选和磁珠分选有什么区别?

    两种细胞分选的区别如下: 1、设施器材:流式分选要准备流式细胞仪等你各种仪器较为复杂;磁珠分选只需要准备磁珠等一起,较为简便。

    2025-01-23 11
  • 免疫荧光定位不对怎么办?

    免疫荧光定位不对怎么办?

    1、细胞核干扰:细胞核位置前面的细胞质染色干扰造成,可以降低抗体浓度,孵育时间 2、细胞或者组织状态不对:细胞或者组织状态不同导致你的目的蛋白细胞

    2025-01-23 8
  • 什么是油红O染色?

    什么是油红O染色?

    油红O染色液主要用于显示组织器官的脂肪变性和类脂质的异常沉着,常发生于肝、肾、心等实质脏器的脂肪变性,细胞内出现多数中性脂肪滴;鉴别和诊断脂肪组

    2025-01-23 10