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Electron Microscopic Studies of Receptor Localization
The localization and density of G-protein-coupled receptors (GPCRs) on the cell
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Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
An important approach in studies of normal, diseased, and malignant cells is the
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Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillar
Within the last few years, capillary electrophoresis (CE), especially with indir
2025-01-24 7 -
Mouse Tissue Culture Models of Unstable Triplet Repeats
Once into the expanded disease-associated range, trinucleotide repeat alleles be
2025-01-24 8 -
Thin-Layer Chromatography of Phospholipids
Thin-layer chromatography (TLC) is a technique that has been routinely used for
2025-01-24 6 -
Fluorescence In Situ Hybridization on Single Cells: Sex Determination and Chromosome Rearrangements
Fluorescence in situ hybridization (FISH) is the technique of choice for preimpl
2025-01-24 7 -
Release Testing of Retroviral Vectors and Gene-Modified Cells
This chapter will review the design and execution of release testing requirement
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A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a
Cleanup technology and mass spectrometric determination of sphingosine-1-phospha
2025-01-24 7 -
裸鼠成瘤肿瘤长不出来怎么办?
答:1、肿瘤生长周期很慢,一周开始生长肿块,大约需要1~2个月才能长成肿瘤。 2、细胞状态差,活力不好,或是肿瘤细胞不易成瘤,需加入
2025-01-23 5 -
做免疫组化时脱片怎么办?
答:1、多聚赖氨酸玻片质量的问题,购买切片时要注意品牌质量。 2、组织切的不好,切片机的问题例如比较老的旧的机器切的厚或者不均匀,
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免疫荧光结果背景强怎么办?
答:免疫荧光背景强是指不能清晰的看到实验目的中的特异性染色,有其他荧光的干扰,影响染色结果的分析和判断。出现荧光背景强的原因和处理如下:
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巨噬细胞纯化的方法有哪些?
1、贴壁法纯化巨噬细胞 (1)用含20%~40%小牛血清的RPMI-1640培养液将巨噬细胞配成2106~4106/ml的浓度。以
2025-01-23 8 -
流式分选和磁珠分选有什么区别?
两种细胞分选的区别如下: 1、设施器材:流式分选要准备流式细胞仪等你各种仪器较为复杂;磁珠分选只需要准备磁珠等一起,较为简便。
2025-01-23 11 -
免疫荧光定位不对怎么办?
1、细胞核干扰:细胞核位置前面的细胞质染色干扰造成,可以降低抗体浓度,孵育时间 2、细胞或者组织状态不对:细胞或者组织状态不同导致你的目的蛋白细胞
2025-01-23 8 -
什么是油红O染色?
油红O染色液主要用于显示组织器官的脂肪变性和类脂质的异常沉着,常发生于肝、肾、心等实质脏器的脂肪变性,细胞内出现多数中性脂肪滴;鉴别和诊断脂肪组
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