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Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
Our laboratory has been engaged in studies of lipid hydrolases since the early 1
2025-01-06 17 -
Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering
Even now, most human cell lines used in research are derived from tumor cells. T
2025-01-06 12 -
Toxicity Methods for CPPs
CPPs have for numerous years been utilized as delivery vectors of various pharma
2025-01-06 11 -
The Latest Improvements in the Mouse Sperm Preservation
Sperm preservation is an important technique for maintaining valuable genetic re
2025-01-06 9 -
Culture of Isolated Human Adipocytes and Isolated Adipose Tissue
Adipose tissue (AT) is no longer considered merely as insulation or padding for
2025-01-06 10 -
Integrin-Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs In Vitro and In Vivo
Utilizing small interfering RNAs (siRNAs) to silence disease-associated genes ho
2025-01-06 14 -
Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Dam
Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher
2025-01-06 12 -
Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
As the equivalent to gatekeepers of the cell, membrane transport proteins perfor
2025-01-06 11 -
Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms
A method is described for measurement of the circadian activity rhythms of up to
2025-01-06 13 -
Raman Microscopy of Bone
Raman microscopy is a non-destructive technique requiring minimal sample prepara
2025-01-06 5 -
Detection of Terminal Transferase in Leukemia
The mere presence of terminal deoxynucleotidyl transferase (TdT), a DNA polymera
2025-01-06 11 -
In Vivo Assay for Tumor Cell Invasion
We describe an in vivo invasion assay that enables the collection of invasive ce
2025-01-06 17 -
Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that init
2025-01-06 5 -
Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic An
The identification and quantitative measurements of proteins in human embryonic
2025-01-06 9 -
MT Spindowns from Extracts
Notes:The key variable in MT spindown experiments is ATP. Under high ATP conditi
2025-01-06 9
