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Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos
Radiolabeled compounds that are substrates for transmembrane transporters can be
2024-12-26 10 -
Methods for Detection of ProteinProteinnl and ProteinDNA Interactions Using HaloTag
HaloTag™ is a protein fusion tag which was genetically engineered to covalently
2024-12-26 16 -
Convergent Extension Analysis in Mouse Whole Embryo Culture
Mutations have been identified in a non-canonical Wnt signalling cascade (the pl
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Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
The gastrointestinal tract of vertebrates is lined by epithelium that develops f
2024-12-26 12 -
Thymic Transplantation in Pig-to-Nonhuman Primates for the Induction of Tolerance Across Xenogeneic
With the advent of knockout pigs for α1,3-galactosyltransferease (GalT-KO, which
2024-12-26 9 -
Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter
Recombinant protein expression in heterologous hosts such as Escherichia coli (E
2024-12-26 8 -
SAPK/Jun kinase assays
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS
2024-12-26 9 -
Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human
The human placenta is a unique and still very poorly understood organ During ges
2024-12-26 13 -
Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells
Although embryonic stem (ES) cells have the regenerative capability of producing
2024-12-26 6 -
Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosectio
MicroRNAs (miRNAs) are 20−25 nucleotide long, noncoding, and single-strand RNAs
2024-12-26 8 -
Isolation and Characterization of Mouse Side Population Cells
The side population (SP) is a subpopulation of mouse bone marrow cells highly en
2024-12-26 16 -
肿瘤发生(tumorigenesis)
正常细胞发展成为肿瘤的过程称为肿瘤发生。从细胞水平上看,癌的发生是极偶然的事件。从遗传上看,癌都是由一个细胞发展而来,由一个失去了增殖控制的细胞发展而来。人体有
2024-12-26 7 -
Tissue Culture of PtK1 cells
Please note: lately (2005 - present), we normally culture PtK1 cells in F-12 med
2024-12-26 6 -
大鼠缺血修饰白蛋白(IMA)酶联免疫分析(ELISA)
大鼠 缺血修饰白蛋白(IMA ) 酶联免疫分析(ELISA )试剂盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体
2024-12-26 8 -
细胞学技术的一般环节简介
相关专题 一、纺锤体阻断剂(Spindle inhibitor) 在有丝分裂过程中,随着纺锤丝的形成,染色体 被牵引到一起难以观察其形态。纺锤体的形成在于细
2024-12-26 9