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  • Measurement of Phosphoinositide 3-Kinase Activity

    Measurement of Phosphoinositide 3-Kinase Activity

    The term “PI 3-kinase” is now applied to a growing family of proteins that are a

    2025-07-08 10
  • Measurement of Phospholipid Metabolism in Intact Neutrophils

    Measurement of Phospholipid Metabolism in Intact Neutrophils

    Phospholipid metabolizing enzymes are important participants in neutrophil signa

    2025-07-08 4
  • Measurement of Proinflammatory Cytokines andT-Cell Proliferative Response in Superantigen-Activated

    Measurement of Proinflammatory Cytokines andT-Cell Proliferative Response in Superantigen-Activated

    The bacterial superantigens, toxic shock syndrome toxin 1 (TSST-1) and the dista

    2025-07-08 10
  • Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry

    Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry

    Stimulation of T lymphocytes, as induced by the interaction of their T-cell rece

    2025-07-08 4
  • Measurement of Respiratory Burst Products Generated by Professional Phagocytes

    Measurement of Respiratory Burst Products Generated by Professional Phagocytes

    Activation of professional phagocytes, potent microbial killers of our innate im

    2025-07-08 5
  • Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP

    Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP

    The development of both microscopic and flow cytometry techniques has allowed in

    2025-07-08 4
  • Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium

    Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium

    This chapter describes assays for the measurement of heme levels and the rate of

    2025-07-08 4
  • Measuring Allergen-Specific IgE: Where Have We Been and Where Are We Going

    Measuring Allergen-Specific IgE: Where Have We Been and Where Are We Going

    About 40 yr ago, two groups of investigators identified a new class of immunoglo

    2025-07-08 4
  • Measuring AntibodyAntigen Binding Kinetics Using Surface Plasmon Resonance

    Measuring AntibodyAntigen Binding Kinetics Using Surface Plasmon Resonance

    Surface plasmon resonance (SPR) is now widely embraced as a technology for monit

    2025-07-08 4
  • Measuring Antimicrobial Peptide Activity on Epithelial Surfaces in Cell Culture

    Measuring Antimicrobial Peptide Activity on Epithelial Surfaces in Cell Culture

    To more accurately assess the activity and role of epithelial cell-derived antim

    2025-07-08 3
  • Measuring Humanized Antibodies in Plasma of Patients Treated With Antibody-Based Therapy Using Bead-

    Measuring Humanized Antibodies in Plasma of Patients Treated With Antibody-Based Therapy Using Bead-

    Alemtuzumab (Campath), the humanized rat monoclonal antibody that targets the CD

    2025-07-08 5
  • Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching

    Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching

    The coordinated effort of cells in the immune system relies heavily on surface r

    2025-07-08 5
  • Measuring Inflammasome Activation in Response to Bacterial Infection

    Measuring Inflammasome Activation in Response to Bacterial Infection

    Inflammasomes are multi-protein signaling platforms assembled in response to vir

    2025-07-08 5
  • Measuring Interferon Alpha and Other Cytokines in SLE

    Measuring Interferon Alpha and Other Cytokines in SLE

    The progression of disease in patients with systemic lupus erythematosus (SLE) i

    2025-07-08 4
  • Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry

    Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry

    This chapter describes a method by which activating receptor-mediated calcium si

    2025-07-08 4