-
Glutaraldehyde-Mediated Protein Immobilization
In this chapter, we describe different approaches for the utilization of glutara
2024-12-20 67 -
Reprogramming of Liver to Pancreas
Islet grafts have demonstrated that patients with diabetes would benefit greatly
2024-12-20 78 -
Assays for Hypermethylation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP
The breast cancer 1 and 2 , early onset (BRCA1 and BRCA2 ) genes are important f
2024-12-20 88 -
Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo
Introducing genes into cells by retroviral transduction has greatly increased th
2024-12-20 87 -
Preparation of tubulin
Although many protocols for tubulin preparation are available, the procedure des
2024-12-20 72 -
Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
Fluorescence live microscopy is a powerful technique to study complex cellular d
2024-12-20 91 -
Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequen
Life expectancy has always been associated to several determinants, such as envi
2024-12-20 88 -
Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatograph
Part A. Sample and Standards Preparation In Chapter 18 , the extraction procedur
2024-12-20 73 -
In Vivo Tumor Formation From Primate Embryonic Stem Cells
To achieve human embryonic stem (ES) cell-based transplantation therapies, allog
2024-12-20 83 -
Embryonic Rodent Brain Cells in Culture
Because of its cellular complexity and regional heterogeneity, the mammalian cen
2024-12-20 85 -
Isolation and Culture of Epithelial Stem Cells
In the skin, epithelial stem cells in the hair follicle contribute not only to t
2024-12-20 71 -
Retroviral Cell Lineage Analysis in the Developing Chick Heart
The use of genetically modified retroviruses as agents for gene transfer has pro
2024-12-20 187 -
Proteomic Analysis of Dictyostelium discoideum
The social amoeba Dictyostelium discoideum is already known as a model organism
2024-12-20 64 -
Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures
In our two-step culturing system, epidermal progenitor cells derived from differ
2024-12-20 98 -
The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity
Recent accumulating data indicate links between planer cell polarity (PCP) and a
2024-12-20 86
