-
Antisense Inhibition: Oligonucleotides, Ribozymes, and siRNAs
Over a span of more than two decades, antisense strategies for gene therapy have
2024-12-20 85 -
细胞表面(cell surface)
细胞表面是一个具有复杂结构的多功能体系。在结构上包括细胞被(cell coat)和细胞质膜。动、植物细胞间的连接结构、细菌与植物细胞的细胞壁以及表面的特化的结构
2024-12-20 111 -
Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embr
We describe in this chapter the development of a xenofree molecularly defined me
2024-12-20 87 -
Activity Assays for Receptor-Interacting Protein Kinase 1:A Key Regulator of Necroptosis
Necroptosis is a novel form of regulated non-apoptotic cell death, which display
2024-12-20 93 -
Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Dro
When you look at the dorsal thorax of a fruitfly, you can easily get fascinated
2024-12-20 84 -
Unbiased Stereological Method to Assess Proliferation Throughout the Subependymal Zone
The subependymal zone (SEZ), frequently named as adult subventricular zone (SVZ)
2024-12-20 98 -
In Situ End-Labeling of Fragmented DNA and the Localization of Apoptosis
Apoptosis is a morphologically distinct form of programmed cell death. It has a
2024-12-20 75 -
Talin and Signaling Through Integrins
Integrin adhesion receptors are essential for the development and functioning of
2024-12-20 91 -
细胞同步化的方法总结
问:在培养细胞取材做其他检测时,为了各个培养瓶中的细胞的齐同性,常须通过同步而实现。具体方法不明,好象是用维持培养液(含较低浓度培养血清),而不是用生长培养液。
2024-12-20 90 -
Review of Human DC Subtypes
Dendritic cells (DC) are critical to the induction and regulation of the innate
2024-12-20 75 -
Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation
Investigation of Granzyme B (GrB) function and pathophysiology in both human set
2024-12-20 79 -
常规组织传代培养
1. 吸除培养瓶内旧培养液。2. 向瓶内加入胰蛋白酶和 EDTA 混合液少许,以能覆满瓶底为限。3. 置温箱中 2~5 分钟,当发现细胞质回缩,细胞间隙增大后,
2024-12-20 90 -
Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors
The tachykinins represent the largest known peptide family and are responsible f
2024-12-20 88 -
Analysis of Nuclear Export Using Photoactivatable GFP Fusion Proteins and Interspecies Heterokaryons
In this chapter, we review protocols for the analysis of nucleocytoplasmic shutt
2024-12-20 95 -
In Vivo Matrigel Migration and Angiogenesis Assays
Angiogenesis, the process of new blood vessels forming from preexisting vessels,
2024-12-20 81