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IN SITU HYBRIDIZATION ON FROZEN SECTIONS
IN SITU HYBRIDIZATION ON FROZEN SECTIONS Protocol by Josiah N. Wilcox, Ph.D.Remo
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The Neverfail Northern Blot Hybridization
The "Neverfail" Northern Blot Hybridization Contributed by James McCau
2024-11-05 29 -
Purification of poly(A)+ RNA
0. (Optional)Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to re
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35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION by Josiah N. Wilcox,
2024-11-05 31 -
Practical Tips for Handling RNA
Phenol ExtractionOne of the most common molecular biology lab procedures, phenol
2024-11-05 26 -
Phenol Extraction of rRNA (Rat liver)
Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFO
2024-11-05 24 -
Water Treatment with DEPC
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave
2024-11-05 28 -
Northern Blotting
Northern BlottingAdapted from Molecular Cloning (Maniatis), Current Protocols in
2024-11-05 32 -
Top Ten Ways to Ensure Valid RNAi Data
１.Confirm results with a second or third siRNA to the same target. One of the
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Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling
2024-11-05 28 -
How to prepare Molecular Biology grade glycogen
How to prepare Molecular Biology grade glycogenAuthor: Roberto SalviSource: Cont
2024-11-05 29 -
Purification of poly(A)+ RNA from total RNA
Purification of poly(A)+ RNA from total RNA0. (Optional)Before using Oligotex-dT
2024-11-05 32 -
RNA interference：The short answer
One way of seeing what a gene does is to block its messenger RNA and note the ef
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RNAi——制备siRNAs的几种方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
2024-11-05 31 -
Preparation of single-stranded probes from cloned cDNA
a) 2-5 µg of plasmid DNA containing the cDNA insert is linearized using an appro
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