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High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed
The overexpression of toxic recombinant proteins is often problematic, leading t
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Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
Clinical grade human mesenchymal stem cells (MSC) are manufactured and used in c
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Microarray Analysis
Microarrays and related technologies have allowed investigators to ask biologica
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Measuring DNA Precursor Pools in Mitochondria
The ability to measure molar concentrations of deoxyribonucleoside 5′-triphospha
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Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
Bioluminescence Resonance Energy Transfer (BRET) measures protein interactions w
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Interface Organotypic Hippocampal Slice Cultures
In complex tissues such as the central nervous system, differentiation and funct
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Freezing Cells
Freezing CellsContributor: Suprya JayadevDate: January 10, 19911) Keep prepare
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肌纤维(myofibers)
肌纤维是组成骨骼肌(skeletal muscle)的肌细胞, 典型的肌细胞是圆柱形的长细胞(长度为1～40mm, 宽为10～100μm), 并且含有许多核(可
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Mutagenesis With Drosophila
Forward genetics in Drosophila has profoundly affected our understanding of circ
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RNA Extraction From Mammalian Tissues
Purification of intact RNA is the primary step of many molecular biology techniq
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PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
RNA localization is an important cellular mechanism for restricting the activiti
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Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
The use of tissue-selective rather than ubiquitous knockouts of steroid receptor
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绒毛染色体制备方法
绒毛来源于胚胎的中胚层，最早的初级干绒毛出现于孕三周初，孕8周左右是绒毛发育最旺盛时期。目前国内外绒毛取材多选于8-9周。研究资料表明，绒毛取样不影响胚胎的发育
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FISH on Plant Chromosomes
FISH, which allows the mapping of DNA sequences directly onto chromosomes, has r
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Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
The following protocols provide a rapid approach for establishing good working c
