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Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
Bioluminescence Resonance Energy Transfer (BRET) measures protein interactions w
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Interface Organotypic Hippocampal Slice Cultures
In complex tissues such as the central nervous system, differentiation and funct
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Freezing Cells
Freezing CellsContributor: Suprya JayadevDate: January 10, 19911) Keep prepare
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肌纤维(myofibers)
肌纤维是组成骨骼肌(skeletal muscle)的肌细胞, 典型的肌细胞是圆柱形的长细胞(长度为1～40mm, 宽为10～100μm), 并且含有许多核(可
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Mutagenesis With Drosophila
Forward genetics in Drosophila has profoundly affected our understanding of circ
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RNA Extraction From Mammalian Tissues
Purification of intact RNA is the primary step of many molecular biology techniq
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PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
RNA localization is an important cellular mechanism for restricting the activiti
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Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
The use of tissue-selective rather than ubiquitous knockouts of steroid receptor
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绒毛染色体制备方法
绒毛来源于胚胎的中胚层，最早的初级干绒毛出现于孕三周初，孕8周左右是绒毛发育最旺盛时期。目前国内外绒毛取材多选于8-9周。研究资料表明，绒毛取样不影响胚胎的发育
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FISH on Plant Chromosomes
FISH, which allows the mapping of DNA sequences directly onto chromosomes, has r
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Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
The following protocols provide a rapid approach for establishing good working c
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Computational Reconstruction of Ancestral DNA Sequences
This chapter introduces the problem of ancestral sequence reconstruction: given
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Characterization of Genes for Polyamine Modulon
Polyamines are essential for normal cell growth and exist mainly as RNA-polyamin
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Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture
Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs
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Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Continuous nucleocytoplasmic shuttling of signal transducer and activator of tra
