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Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides
The isolation of uncontaminated, intact RNA is essential for analyzing gene expr
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Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
The ideal way to assess hematopoietic stem cells is to observe their growth in t
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Handmade Somatic Cell Cloning in Cattle
Apart from the biological and ethical problems, technical difficulties also hamp
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Drosophila melanogaster and the Development of Biology in the 20th Century
The fruit fly Drosophila has played a central role in the development of biology
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A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein
A large number of proteins contain multiple RNA recognition motifs (RRMs). How m
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RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
Reverse transcription and the polymerase chain reaction (RT-PCR) provides a very
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Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium
Several distinct stages of differentiation have been described for the oligodend
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Assay of Deoxyhypusine Synthase Activity
Deoxyhypusine synthase catalyzes an unusual protein modification reaction. A por
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核孔运输(transport through nuclear pore)
胞质溶胶中合成的蛋白质穿过细胞核内外膜形成的核孔进入细胞核。核孔运输又称为门运输,核孔是如同一扇可开启的大门,而且是具有选择性的门,能够主动运输特殊的生物大分子
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Electrotransfection with Intracellular Buffer
Introduction of foreign molecules, such as DNA, RNA, or proteins, into living ce
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Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
Many cell cycle transitions are controlled by ubiquitin-mediated proteolysis of
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Effects of Rho Family GTPases on Cell-Cell Adhesion
Cell-cell adhesion is dynamically rearranged in various cellular processes, incl
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中心体
中心体存在于低等植物细胞(如衣藻、团藻等藻类植物)和动物细胞中。中心体不具备膜结构,是由蛋白质组成的。每个中心体是由两个互相垂直的短棒状的中心粒排列而成,每个中
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Modulation of Potassium Ion Channel Proteins Utilising Antibodies
The application of antibodies to living cells has the potential to modulate the
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Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Ubiquitination and protein degradation regulate cell cycle progression in all eu