-
Generation of Transgene-Free iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal
Human induced pluripotent stem cells (iPSCs) have become an important tool for m
-
Developmental Biology Protocols: Overview I
As the next millennium dawns, developmental biology, the study of the processes
-
Immunogold Labelling for Scanning Electron Microscopy
Scanning electron microscopes are useful biological tools that can be used to im
-
Human Long-Term Culture Initiating Cell Assay
The long-term culture initiating cell (LTC-IC) assay, founded on the bone marrow
-
Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Li
Depletion of pancreatic acinar cell polyamines in response to activation of poly
-
Electroporation Strategies for Genetic Manipulation and Cell Labeling
Electroporation has emerged as an effective method for cell labeling and manipul
-
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
Liver regenerates after hepatectomy or chemical-induced injury. In contrast to c
-
Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
Marrow stromal cells (MSCs) are relatively rare cells difficult to visualize in
-
Transplantation of Human Embryonic Stem Cells to the Chick Embryo
The traditional methods of studying the differentiation of human embryonic stem
-
Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography,
Various analytical techniques have been adopted for the isolation and identifica
-
缝隙连接(gap junction)
缝隙连接分布广泛,几乎存在于所有的动物细胞中。用超薄切片术可显示相邻两细胞的连接处的细胞质膜明暗相间七层结构,细胞间的缝隙约2纳米,其内有间隔的均匀排列的颗粒
-
Microinjection into Xenopus Oocytes and Embryos
The ease of obtaining large numbers of Xenopus laevis eggs and oocytes together
-
M-Current Recording from Acute DRG Slices
Electrophysiological recordings from an acutely sliced preparation provide infor
-
前质体(proplastid)
前质体是质体的前身, 仅由内膜和外膜构成,膜内是少量的基质和DNA,但是没有LHC蛋白、叶绿素和电子传递系统。植物中的前质体随着在发育过程中所处的位置以及接受光
-
The Problems and Pitfalls of Radioligand Binding
Radioligand binding is an extremely versatile technique that can be applied to a