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Purification of Murine Monoclonal Antibodies
Hybridoma technology has made possible the production of highly specific, homoge
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Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Immobilization is designed to restrict the freedom of movement of an enzyme and,
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Oocyte Isolation and Enucleation
Xenopus laevis oocytes are popular cells in experimental biology. Fully grown oo
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Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein
The cytoskeleton is a dynamic structure comprised of at least three distinct cel
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Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Whole-mount in situ hybridization (WISH) is a reliable and specific method to st
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Detection of Apoptosis in the Central Nervous System
Apoptosis occurs in the nervous system during normal development, but can also b
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Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Vectors proposed for gene delivery generally fall into two categories: viral and
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Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell
Hematopoietic progenitor and stem cells are believed to lie dormant within the a
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Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
Prolonged mechanical loading of soft tissues may result in degeneration of these
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Epithelial-Mesenchymal Interactions
Cell-cell and tissue-tissue interactions initiate and regulate genomic expressio
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自由基(free radical)
自由基是指那些在原子核外层轨道上具有不成对电子的分子或原子基团。所谓未成对电子,就是指那些在原子或分子轨道中未与其它电子配对而独占一个轨道的电子。如AB两个原子
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DNA Microarray for Molecular Epidemiology of Salmonella
Salmonellosis is a common infection estimated to affect 3 billion people and to
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Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion
Cell fusion is a powerful tool for understanding the molecular mechanisms of epi
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Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis
Before the advent of polymerase chain reaction (PCR) technology, many methods fo
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液泡(vacuoles)
植物中由膜包裹的结构, 几乎占据了细胞总体积的90%。植物细胞的液泡也含有多种水解酶类, 具有与动物细胞溶酶体酶类似的功能。液泡膜上具有H -ATPase, 能
