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Canine Models of Gene-Modified Hematopoiesis
Large animal models have played a crucial role in the development of gene therap
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Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
Over the past decade, a variety of oligonucleotide-based probes have been develo
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Isolation and Culture of Mouse Intestinal Cells
Complex cell signal transduction mechanisms regulate intestinal epithelial shape
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Examination of Normal and Abnormal Placentation in the Mouse
Placental development, also known as placentation, is orchestrated by precise mo
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流式细胞仪主要技术指标
1 ．流式细胞仪的分析速度： 一般流式细胞仪每秒检测 1000 ～ 5000 个细胞 , 大型机可达每秒上万个细胞。 2 ．流式细胞仪的荧光检测灵敏度：一般能测
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Transfection and Transformation of Human Thyroid Epithelial Cells
High efficiency gene transfer into mammalian fibroblasts is a routine procedure
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细胞粘着(cell adhesion)
在细胞识别的基础上, 同类细胞发生聚集形成细胞团或组织的过程叫细胞粘着。它对于胚胎发育及成体的正常结构和功能都有重要的作用。在发育过程中,由于细胞间细胞粘着的强
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冰冻断裂(freeze fracture)
一种制备电子显微镜样品的方法。将组织放在液氮中快速下冷冻，然后用冰刀使样品断裂分割，通过金属复形可进行电镜观察。
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Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
Proliferation, migration, and invasion of trophoblastic cells into the maternal
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Preparation and Analysis of Uniquely Positioned Mononucleosomes
Short DNA fragments containing single, uniquely positioned nucleosome cores have
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Screening of Telomerase Inhibitors
Shortening of telomeres prevents cells from uncontrolled proliferation. Progress
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Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Gerany
Rab proteins are small Ras-like GTPases that regulate vesicular trafficking even
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核仁周期(nucleolar cycle)
核仁是一种动态结构，随细胞周期的变化而变化，即形成�消失�形成,这种变化称为核仁周期。在细胞的有丝分裂期，核仁变小，并逐渐消失。在有丝分裂末期，rR
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The Introduction of Proteins into Mammalian Cells by Electroporation
Proteins can be introduced into cells in several ways, including permeabilizatio
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PCR Sequencing with the Aid of Detergents
The polymerase chain reaction (PCR) allows the rapid detection of infectious vir
