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Nuclear Transplantation in Xenopus
Nuclear transplantation in amphibia started in 1952. By this is meant, sexually
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Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization
Although recent molecular biology advances provide a very effective way in deter
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Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
This chapter will focus on responses that the chemoattractant cyclic AMP elicits
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Analysis of B-Cell Life-Span and Homeostasis
In the past, the life-span of B cells in rodents has been determined by a variet
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Detergent-Extracted Models for the Study of Cilia or Flagella
The plasma membrane of the living cell is designed to regulate the internal comp
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The Voltage-Clamp Fluorometry Technique
Ion channels are the cell's gatekeepers. These proteins selectively allow io
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Remote Loading of Anthracyclines into Liposomes
The following chapter introduces a remote loading procedure for anthracyclines f
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Cell Lineage Analysis: Applications of Green Fluorescent Protein
The ideal cell lineage marker is one that can be visualized in living tissues wi
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Microtubule Binding Assays
MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X
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Detection of Nuclear Envelope Alterations in Senescence
Gene mutations that cause defects in the nuclear envelope are responsible for pr
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A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction
The generation of nested deletions within cloned fragments of DNA has important
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母体信息(maternal information)
卵母细胞带来的信息分子称为母体信息。据分析, 许多动物的卵母细胞中含有2~5万种不同核苷酸顺序的mRNA,每种有600个拷贝之多。据推测,这些母体信息在受精前都
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In situ Hybridization with RNA Probes
Immunohistochemistry or immunofluorescence analyses are ideal techniques for loc
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Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological condi
Immunohistochemistry or immunofluorescence analyses are ideal techniques for loc
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Immunostaining of Skeletal Tissues
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis o