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红细胞比容(hematocrit value)
指红细胞占全血容积的百分比。它反映红细胞和血浆的比例。将已加少量抗凝剂混匀的血液,装入特制的、具刻度的比容管,以每分钟3000转的速度离心半小时,可见血液各
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Growing feeder-independent embryonic stem cells§
We use feeder-independent ES cell lines derived from the 129/Ola strain of mice
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Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
Compromised mitochondrial DNA structural integrity can have functional consequen
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Tumor Cell Invasion Assays
The ability of tumor cells to invade is one of the hallmarks of the metastatic p
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A Novel Method for the Production of Fully Modified K-Ras 4B
Post-translational modifications in proteins play a major functional role. Post-
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Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription
Genome-wide screens have proven powerful in associating gene products with certa
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Chemotaxis Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or
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酸性磷酸酶法检验细胞增殖
1.96孔细胞培养板中培养细胞,去培养液。用0.01mol/L PBS洗涤1次(如为悬浮细胞则用离心洗涤)。 2.去洗涤液后加入磷酸酶底物100μl/孔。 3.
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Retroviral Gene Transduction in Limb Bud Micromass Cultures
Since Solursh and his coworkers set up the limb bud micromass cultures in 1977 (
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Retroviral Integration Site Analysis in Hematopoietic Stem Cells
Stable transgene insertion into a host genome irrevocably and unambiguously mark
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Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation
The study of metalloproteins by resonance Raman (RR) spectroscopy began two deca
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Canine Models of Gene-Modified Hematopoiesis
Large animal models have played a crucial role in the development of gene therap
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Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
Over the past decade, a variety of oligonucleotide-based probes have been develo
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Isolation and Culture of Mouse Intestinal Cells
Complex cell signal transduction mechanisms regulate intestinal epithelial shape
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Examination of Normal and Abnormal Placentation in the Mouse
Placental development, also known as placentation, is orchestrated by precise mo