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Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis
Human cytomegalovirus (CMV) is a ubiquitous pathogen found in 40–100% of adults,
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Detection of Human and Mouse Granzyme B Activity in Cell Extracts
The serine protease granzyme B (GrB) is a key effector molecule in cell-mediated
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Cloning PCR Products for Sequencing in M13 Vectors
Although numerous methods are now available for direct sequencing of PCR product
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Isolation of Neuroepithelium and Formation of Minispheres
The brain of the early chick embryo initially grows by expansion of its fluid-fi
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Culture of Human Renal Medullary Interstitial Cells
The renal medullary interstitial cell (RMIC) is a unique lipid containing cell f
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Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluoresce
The combined use of fluorescence microscopy with atomic force microscopy (AFM) h
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Designing and Utilization of siRNAs Targeting RNA Binding Proteins
Small interfering RNA (siRNA)-mediated RNA interference (RNAi) is a very powerfu
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Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants
Fluorescence tagging of genomic sites through the use of bacterial operator/repr
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Immunofluorescence Methods in Studies of the GTPase Ran and Its Effectors in Interphase and in Mitot
The GTPase RAN, the regulators of its nucleotide-bound state, and its effectors
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Cryopreservation of the Sperm of the Pacific Oyster Crassostrea gigas
In contrast to the extensive research and advances in the cryopreservation of an
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Basic Analyses of Proteins of Interest for Xenotransplantation
Proteins are the focus of numerous xenotransplantation studies because they prov
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Wingless Signaling in Drosophila Eye Development
The secreted morphogen Wingless (Wg) has a variety of functions throughout Droso
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A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene
Small non-coding RNAs (sRNAs) are an emerging class of regulators of bacterial g
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Protein Separation Using Immobilized Phospholipid Chromatography
The chromatographic support containing monolayers of phospholipids offers novel
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Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism
Polyamines are intimately linked to essential cellular processes that are requir
