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Visualization of mRNA Expression in the Zebrafish Embryo
Examination of spatial and temporal gene expression pattern is a key step toward
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Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines
For more than one decade patients have been treated with dendritic cell (DC) imm
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Fourier Transform Infrared Imaging of Bone
Fourier transform infrared imaging (FTIRI) is a technique that can be used to an
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Transient Overexpression of Genes in Neurons Using Nucleofection
Nucleofection is a transfection method used to introduce substrates such as cDNA
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Isolation and Culture of Mouse Satellite Cells
Muscle tissue culture provides a system for studying the growth and differentiat
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Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues
This section focuses on the application of differential display in gaining insig
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Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes
The primary method for determining the function of a gene in rodents has been to
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Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells
The common procedures that are used to quantify cyclobutane pyrimidine dimers (C
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HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids
Eicosanoids are oxygenated, endogenous, unsaturated fatty acids derived from ara
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初级溶酶体(primary lysosome)
此类溶酶体是刚刚从反面高尔基体形成的小囊泡, 仅含有水解酶类,但无作用底物,外面只有一层单位膜,其中的酶处于非活性状态。如果从细胞的分泌活动考虑,初级溶酶体是一
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Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements
Adipocytes express the three major types of adrenoceptors (ARs), α1 -, α2 -, and
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Role of Endothelial CellCell Junctions in Endothelial Permeability
The endothelial barrier separates the inner blood compartment from the surroundi
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Purification of Plant Ribosomal DNA: Use of Buoyant Density Centrifugation in Cesium Chloride-Actino
The general features of isopycnic centrifugation of DNA and the separation of ma
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Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports
Immobilization of enzymes and cells has been widely investigated for many years,
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Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Mic
The detailed understanding of the DNA replication process requires structural in