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Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mas
Due to the complexity of the mammalian central nervous system, neuropeptidomic s
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Generation of Tissue Organoids by Compaction Reaggregation
Cellular reaggregation methods are commonly used to generate tissue organoids fo
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Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma
Maltose-binding protein (MBP) is a carrier protein for high level recombinant pr
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Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)
Current sources of mesenchymal cells, including bone marrow, fat and muscle, all
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Murine Bone Marrow-Derived Macrophages
The molecular phagocytic lineage comprises, in order of increasing maturity, the
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纺锤体(spindle)
大量微管纵向排列组成的中间宽两极小的细胞器,形状象纺锤,因而得名。因为纺锤体和染色体运动密切相关,所以纺锤体是一个重要的有丝分裂装置。纺锤体由极间丝(又叫连续
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Sos蛋白(Sos protein)
Sos蛋白是编码鸟苷释放蛋白的基因sos的产物(sos是son of sevenless 的缩写)。Sos蛋白在Ras信号转导途径中的作用是促进Ras释放GDP
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Melanosome Motility in Fish Retinal Pigment Epithelial Cells
Several model systems have been developed to investigate intracellular organelle
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Visualization of Induced RNA in Single Bacterial Cells
Visualization of RNA in live cells is a challenging task due to the transient ch
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Isolation and Short-Term Culture of Human Epidermal Langerhans Cells
The human epidermis consists of a heterogeneous population of cells including ke
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Visualization of Spindle Behavior Using Confocal Microscopy
Numerous comparisons have shown that confocal imaging of fluorescently labeled s
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Yeast Protoplast Fusion
A major limitation to the results that could be achieved using traditional metho
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Immobilization of Enzymes and Proteins on Red Blood Cells
Immobilization of proteins on intact red blood cells (RBCs) can be valuable for
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The Measurement of Protein Degradation in Response to Oxidative Stress
Molecular oxygen is an excellent acceptor of electrons and is therefore employed
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Cell Viability Assays: Introduction
The measurement of cell viability plays a fundamental role in all forms of cell